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CoraLite® Plus 647 Anti-Human CD107a / LAMP1 (H4A3)

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4A3

Cat No : CL647-65051

Print datasheet

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

验证数据展示

  • FC experiment of HeLa using CL647-65051

    FC experiment of HeLa using CL647-65051

    1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a (CL647-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL647-65051

    IF Staining of HeLa using CL647-65051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107a antibody (CL647-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 65051-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

产品信息

CL647-65051 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF
Tested Reactivity Human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker
GenBank accession numberBC006345
Gene symbol LAMP1
UniprotP11279
Gene ID (NCBI) 3916
RRIDAB_2883654
Conjugate CoraLite® Plus 647 Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65051Download protocol
FC protocol for CL Plus 647 CD107a / LAMP1 antibody CL647-65051Download protocol
Standard Protocols
Click here to view our Standard Protocols