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Ago4 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 39813,39814 39813

Synonyms

ago, argonaute, dicer, rnase, rnai, silencing, sirna, mirna, pri-mirna, risc, rna interference, ago4, argonaute 4, ago 4, argonaute 4, PTSG, sample

验证数据展示

  • Ago4 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 ug) probed with Ago4 antibody at a dilution of 1:1,000.

    Ago4 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 ug) probed with Ago4 antibody at a dilution of 1:1,000.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Ago4 antibody was raised against a peptide within the N-terminal region of human Ago4.
Full Name Ago4 antibody (pAb)
Synonyms ago, argonaute, dicer, rnase, rnai, silencing, sirna, mirna, pri-mirna, risc, rna interference, ago4, argonaute 4, ago 4, argonaute 4, PTSG, sample
Molecular weight 100 kDa
GenBank accession numberNP_060099
RRIDAB_2793351
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.