CoraLite® Plus 488-conjugated CYP3A4 Monoclonal antibody
CYP3A4 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F11C4
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CYP3A4 Ready-To-Use IHC Kit. CYP3A4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67110-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67110 targets CYP3A4 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | CYP3A4 fusion protein Ag13074 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| Synonyms | Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase |
| Calculated Molecular Weight | 503 aa, 57 kDa |
| Observed Molecular Weight | 51-57 kDa |
| GenBank Accession Number | BC101631 |
| Gene Symbol | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08684 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The murine homolog of human CYP3A4 is CYP3A11 (PMID: 19297428). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CYP3A4 antibody CL488-67110 | Download protocol |
| FC protocol for CL Plus 488 CYP3A4 antibody CL488-67110 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
