CYP3A4 Monoclonal antibody
CYP3A4 Monoclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig and More (1)
Applications
IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2F11C4
验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, pig liver tissue, pig gall bladder tissue, mouse lung tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse liver tissue, rat liver tissue, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CYP3A4 Ready-To-Use IHC Kit. CYP3A4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
67110-1-Ig targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human |
| Immunogen | CYP3A4 fusion protein Ag13074 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 3, subfamily A, polypeptide 4 |
| Synonyms | Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase |
| Calculated Molecular Weight | 503 aa, 57 kDa |
| Observed Molecular Weight | 51-57 kDa |
| GenBank Accession Number | BC101631 |
| Gene Symbol | CYP3A4 |
| Gene ID (NCBI) | 1576 |
| RRID | AB_2882414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IHC protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| IF protocol for CYP3A4 antibody 67110-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Rep Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells. | ||
Methods Mol Biol Homogeneous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells. | ||
Acta Biomater Hepar-on-a-sensor-platform with hybridization chain reaction amplification strategy to intuitively monitor the hepatoxicity of natural compounds |
