HER2/ErbB2 Monoclonal antibody

HER2/ErbB2 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B12A7

Cat No : 60311-1-Ig

Print datasheet

Synonyms

ERBB2, HER2, HER 2/neu, HER 2, CD340



经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60311-1-Ig targets HER2/ErbB2 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen HER2/ErbB2 fusion protein Ag16463 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Synonyms ERBB2, HER2, HER 2/neu, HER 2, CD340
Calculated Molecular Weight 1255 aa, 138 kDa
GenBank Accession NumberBC156755
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
RRIDAB_2881423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HER2, ErbB2, and Neu is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.

实验方案

Product Specific Protocols
IHC protocol for HER2/ErbB2 antibody 60311-1-IgDownload protocol
IF protocol for HER2/ErbB2 antibody 60311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Cancer

InvivoPen: A novel plasma source for in vivo cancer treatment.

Authors - Xin Zhou
humanIHC

Mol Med Rep

HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma‑not otherwise specified.

Authors - Jiaojiao Wang
humanIHC

Oncol Lett

Circulating HER-2 mRNA in the peripheral blood as a potential diagnostic and prognostic biomarker in females with breast cancer.

Authors - Yanlin Wu
humanIF

Mol Cancer

CMTM6 overexpression confers trastuzumab resistance in HER2-positive breast cancer

Authors - Fei Xing
humanIHC

Int J Cancer

Establishment of patient-derived organoids for guiding personalized therapies in breast cancer patients

Authors - Huizi Wu