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  • KD/KO Validated

EGFR-Specific Polyclonal antibody

EGFR-Specific Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

FC, IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 18986-1-AP

Print datasheet

Synonyms

EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1



经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, MCF-7 cells, multi-cells, HeLa/RPE1 cells, HeLa cells, NCI-H1299 cells, A549 cells, L02 cells, NCL-H1299 cells
Positive IP detected inMCF-7 cells
Positive IF detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18986-1-AP targets EGFR-Specific in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, sheep, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Synonyms EGFR, EGFR-Specific, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1
Calculated Molecular Weight 134 kDa
Observed Molecular Weight 145-165 kDa
GenBank Accession NumberNM_005228
Gene Symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_10596476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.

实验方案

Product Specific Protocols
WB protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IF protocol for EGFR-Specific antibody 18986-1-APDownload protocol
IP protocol for EGFR-Specific antibody 18986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
  • KD Validated
humanWB

Cancer Commun (Lond)

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

Authors - Xiaoteng Cui
humanIF

ACS Appl Mater Interfaces

On-Chip Replication of Extremely Early-Stage Tumor Behavior.

Authors - Chengpan Li
pigWB

Biosens Bioelectron

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic.

Authors - Yi-Jyun Chen
humanWB, IHC, ChIP

Cancer Res

Hypoxia Alters the Response to Anti-EGFR Therapy by Regulating EGFR Expression and Downstream Signaling in a DNA Methylation-Specific and HIF-Dependent Manner.

Authors - Mahelet Mamo
WB, IF

Curr Biol

Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.

Authors - Nazim Ali