CoraLite® Plus 488-conjugated HER2/ErbB2 Monoclonal antibody
HER2/ErbB2 Monoclonal Antibody for FC, IF-P
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
FC, IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B12A7
验证数据展示
经过测试的应用
| Positive IF detected in | human breast cancer tissue |
| Positive FC detected in | SK-BR-3 cells |
For other applications, we recommend the unconjugated version of this antibody, 60311-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60311 targets HER2/ErbB2 in IF, FC applications and shows reactivity with human samples.
| Tested Applications | FC, IF-P |
| Tested Reactivity | human |
| Immunogen | HER2/ErbB2 fusion protein Ag16463 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Synonyms | CD340, ERBB2, HER 2, HER 2/neu, HER2, HER2/ErbB2, MLN 19, MLN19, NEU, NGL, p185, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, TKR1 |
| Calculated Molecular Weight | 1255 aa, 138 kDa |
| GenBank Accession Number | BC156755 |
| Gene Symbol | ERBB2 |
| Gene ID (NCBI) | 2064 |
| RRID | AB_2883140 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04626 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including
breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HER2/ErbB2 antibody CL488-60311 | Download protocol |
| FC protocol for CL Plus 488 HER2/ErbB2 antibody CL488-60311 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
