Various lysates were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
IP result of anti-MICAL1 (IP:68297-1-Ig, 4ug; Detection:68297-1-Ig 1:2000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MICAL1 antibody (68297-1-Ig, Clone: 3F5D4 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68297-1-Ig (MICAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed A549 cells using MICAL1 antibody (68297-1-Ig, Clone: 3F5D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MICAL1 (68297-1-Ig, Clone:3F5D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68297-1-PBS in a different storage buffer formulation.