Featured Product
KD/KO Validated

MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat No : 14818-1-AP

Print datasheet

Synonyms

MICAL, MICAL 1, MICAL1, NICAL

验证数据展示

WB analysis of HeLa using 14818-1-AP
WB result of MICAL1 antibody (14818-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICAL1 transfected HeLa cells.
IF Staining of HeLa using 14818-1-AP
IF result of MICAL1 antibody (14818-1-AP) with Hela Cells (Methanol fixation) MICAL1 in Red (endogenous) and BioGFP-MICAL1-FL overexpression. Courtesy of Qingyang Liu, Utrecht University.
WB analysis of Jurkat using 14818-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14818-1-AP
IP result of anti-MICAL1 (IP:14818-1-AP, 5ug; Detection:14818-1-AP 1:1000) with HeLa cells lysate 2000ug.
IHC staining of human lung using 14818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 14818-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).
FC experiment of HeLa using 14818-1-AP
1X10^6 HeLa cells were stained with 0.2ug MICAL1 antibody (14818-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IF Staining of A549 using 14818-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using MICAL1 antibody (14818-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

-/ -

Cat No. 14818-1-AP

Size

点击咨询在线客服

经过测试的应用

Positive WB detected in	Jurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue
Positive IP detected in	HeLa cells, T-47D cells
Positive IHC detected in	human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A549 cells, HeLa cells
Positive FC detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:200-1:800
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 10 publications below
WB	See 13 publications below
IHC	See 5 publications below
IF	See 7 publications below
CoIP	See 1 publications below

产品信息

14818-1-AP targets MICAL1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	CoIP, IF, IHC, WB
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Immunogen	MICAL1 fusion protein Ag6578 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	microtubule associated monoxygenase, calponin and LIM domain containing 1
Synonyms	MICAL, MICAL 1, MICAL1, NICAL
Calculated Molecular Weight	118 kDa
Observed Molecular Weight	120 kDa
GenBank Accession Number	BC052983
Gene Symbol	MICAL1
Gene ID (NCBI)	64780
RRID	AB_2143754
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TDZ2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

实验方案

Product Specific Protocols
WB protocol for MICAL1 antibody 14818-1-AP	Download protocol
IHC protocol for MICAL1 antibody 14818-1-AP	Download protocol
IF protocol for MICAL1 antibody 14818-1-AP	Download protocol
IP protocol for MICAL1 antibody 14818-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Sci Adv F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis. Authors - Fengfeng Niu KO Validated View Article
human	WB	Nat Commun Oxidation of F-actin controls the terminal steps of cytokinesis. Authors - Stéphane Frémont KD Validated View Article
human	CoIP	Dev Cell Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling. Authors - Jimok Yoon View Article
human	WB	J Cell Biol MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes. Authors - Chiara Galloni KD Validated View Article
human	IHC	Oncogene Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement Authors - Ze Zhang View Article
		Diabetes Semaphorin3a promotes advanced diabetic nephropathy. Authors - Pardeep K Aggarwal View Article

MICAL1 Polyclonal antibody

MICAL1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis of HeLa using 14818-1-AP

IF Staining of HeLa using 14818-1-AP

WB analysis of Jurkat using 14818-1-AP

IP experiment of HeLa using 14818-1-AP

IHC staining of human lung using 14818-1-AP

IHC staining of human lung using 14818-1-AP

FC experiment of HeLa using 14818-1-AP

IF Staining of A549 using 14818-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.

Oxidation of F-actin controls the terminal steps of cytokinesis.

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement

Semaphorin3a promotes advanced diabetic nephropathy.