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Validation Data Gallery

  • Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human BDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

    BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

  • iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

    iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

  • Proteintech BDNF (Cat no: HZ-1335) demonstrates equivalent fold induction and a greater than 5-fold increase in potency compared to leading competitors. BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

    Proteintech BDNF (Cat no: HZ-1335) demonstrates equivalent fold induction and a greater than 5-fold increase in potency compared to leading competitors. BDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant BDNF for 72 hours after an initial 5 day incubation with 10 μM retinoic acid. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 100 ng/mL.

  • Differentiation of SHSY5Y cells into neurons using HumanKine BDNF in the presence of Retinoic acid. The data demonstrates significantly better neuronal differentiation using HumanKine BDNF compared to leading competitor in the market.

    Differentiation of SHSY5Y cells into neurons using HumanKine BDNF in the presence of Retinoic acid. The data demonstrates significantly better neuronal differentiation using HumanKine BDNF compared to leading competitor in the market.

  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BDNF protein

Cat No. HZ-1335
引用文献(5)

Activity

4-40 ng/mL

Specific Activity

minimally 3.00 x105 IU/mg

Species Reactivity

human

Purity

>95%

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor

Product type:  Research use only
规格价格库存

GMP-Grade HumanKine® Info

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Technical Specifications

GeneID 627
Species Human
Expression HEK293
Activity 4-40 ng/mL
Specific Activityminimally 3.00 x105 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP23560
Molecular Mass 14 kDa reduced and non-reduced, homodimer, non-glycosylated
Formulation 10 x PBS pH 7.4 See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

HumanKine® Brain-Derived Neurotrophic Factor (BDNF) is a key neurotrophin that supports neuronal survival, differentiation, and maturation in the central and peripheral nervous systems. Highly expressed in the hippocampus, cortex, cerebellum, and amygdala, BDNF is widely used as a cell culture supplement for neural differentiation, brain organoid development, and stem cell research. Produced in a HEK293 human expression system, HumanKine® BDNF closely mimics native human protein, offering enhanced bioactivity and stability. BDNF promotes axonal growth, dendritic development, synaptic transmission, and plasticity by activating the TrkB receptor, a pathway essential for learning and memory. BDNF also supports angiogenesis and endothelial cell survival. Reduced BDNF levels are linked to neurodegenerative disorders including Parkinson’s disease, multiple sclerosis, Huntington’s disease, and autism.

Synonyms

Abrineurin, ANON2, brain-derived neurotrophic factor

Publications

SpeciesTitle
mouse

Mol Metab

Crosstalk between the muscular estrogen receptor α and BDNF/TrkB signaling alleviates metabolic syndrome via 7,8-dihydroxyflavone in female mice.

Authors - Zhenlei Zhao
View Article
human

Hum Cell

Establishment and characterization of ZJUCHi003: an induced pluripotent stem cell line from a patient with Temple-Baraitser/Zimmermann-Laband syndrome carrying KCNH1 c.1070G > A (p.R357Q) variant

Authors - Die Chen
View Article
mouse

Adv Sci (Weinh)

Integration of Functional Human Auditory Neural Circuits Based on a 3D Carbon Nanotube System

Authors - Yiyun Lou
View Article
rat

Biomed Pharmacother

Intratympanic injection of MSC-derived small extracellular vesicles protects spiral ganglion neurons from degeneration

Authors - Anning Chen
View Article
mouse

Sci Rep

BDNF improves fracture healing through promoting osteoblasts proliferation and migration via trkb/akt regulated NUCKS1 expression

Authors - Weihao Meng
View Article
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