Validation Data Gallery
Technical Specifications
| GeneID | 3606 |
| Species | Human |
| Expression | HEK293 |
| Activity | 20 -160 ng/mL |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | Q14116 |
| Molecular Mass | 19 kDa reduced, 19-21 kDa non-reduced, monomer, non-glycosylated |
| Formulation | 1X PBS pH 7.4 + 0.5% CHAPS |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Human Interleukin-18 (IL-18) is a pro-inflammatory cytokine from the IL-1 family and a critical mediator of innate and adaptive immunity. IL-18 functions primarily by inducing IFN-γ production, supporting Th1 polarization, enhancing NK-cell cytotoxicity, and cooperating with IL-12 in pathogen defense and inflammatory signaling. IL-18 is broadly expressed as an inactive precursor in various tissues and cell types, especially those involved in immune surveillance and barrier defense. IL-18 is synthesized as pro-IL-18 and requires caspase-1 (inflammasome) processing for activation. This two-step regulation restricts its biological activity to inflammatory contexts while allowing broad basal expression.
Synonyms
IGIF, IL1F4
