- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human GM-CSF protein- GMP grade
EC50
0.08-0.8 ng/mL EC50
Specific Activity
>6.00x106 IU/mg
Species Reactivity
human,pig,mouse
Purity
>95%
![ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors](/img/GMP-LOGO.jpg)
Cat No : HZ-1002-GMP
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Validation Data Gallery
Technical Specifications
GeneID | 1437 |
Species | Human |
Expression | HEK293 |
EC50 | 0.08-0.8 ng/mL EC50 |
Specific Activity | >6.00x106 IU/mg |
Purity | >95% |
Endotoxin | <0.1 EU/µg |
Accession Number | P04141 |
Molecular Mass | 15 to 36 kDa reduced and non-reduced, monomer, glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,pig,mouse |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. Reconstitution is recommended at 0.2 mg/mL using sterile 1 x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
GMP Quality Policies
HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Publications
Species | Title |
---|---|
Oral Dis Notch activation promotes bone metastasis via SPARC inhibition in adenoid cystic carcinoma |