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WT1 Recombinant antibody

WT1 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2J13

Cat No : 82525-1-RR

Print datasheet

Synonyms

GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33

验证数据展示

  • WB analysis using 82525-1-RR

    WB analysis using 82525-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 82525-1-RR

    IHC staining of rat testis using 82525-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82525-1-RR targets WT1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen WT1 fusion protein Ag19541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wilms tumor 1
Synonyms GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT 2, WT1, WT33
Calculated Molecular Weight 449 aa, 49 kDa, 57 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.

实验方案

Product Specific Protocols
WB protocol for WT1 antibody 82525-1-RRDownload protocol
IHC protocol for WT1 antibody 82525-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols