• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UBE2E3 Polyclonal antibody

UBE2E3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15488-1-AP

Print datasheet

Synonyms

UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3

验证数据展示

  • WB analysis of Jurkat using 15488-1-AP

    WB analysis of Jurkat using 15488-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15488-1-AP (UBE2E3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 15488-1-AP

    IHC staining of human heart using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 15488-1-AP

    IHC staining of human heart using 15488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15488-1-AP (UBE2E3 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, mouse kidney tissue, mouse liver tissue, Y79 cells
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15488-1-AP targets UBE2E3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen UBE2E3 fusion protein Ag7838 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Synonyms UBCE4, UBCH9, UbcM2, UBE2E3, Ubiquitin carrier protein E3, Ubiquitin protein ligase E3
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-27 kDa
GenBank Accession NumberBC003554
Gene Symbol UBE2E3
Gene ID (NCBI) 10477
RRIDAB_10896456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969T4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UBE2E3 antibody 15488-1-APDownload protocol
IHC protocol for UBE2E3 antibody 15488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Neuropathology

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

Authors - Kazuhiko Watabe
View Article
mouseWB

Int J Mol Sci

miR-143-3p Promotes Ovarian Granulosa Cell Senescence and Inhibits Estradiol Synthesis by Targeting UBE2E3 and LHCGR

Authors - Jingxian Deng
View Article