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Transgelin-2-specific Polyclonal antibody
Transgelin-2-specific Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IP detected in | L02 cells |
Positive IHC detected in | human oesophagus cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15508-1-AP targets Transgelin-2-specific in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transgelin 2 |
Synonyms | TAGLN2, Transgelin 2, Transgelin-2-specific |
Calculated Molecular Weight | 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | NM_003564 |
Gene Symbol | TAGLN2 |
Gene ID (NCBI) | 8407 |
RRID | AB_2199501 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P37802 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IHC protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IF protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
IP protocol for Transgelin-2-specific antibody 15508-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.
| ||
J Gastroenterol Hepatol The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma. | ||
Cancer Cell Int MiR-613 inhibits the proliferation, migration, and invasion of papillary thyroid carcinoma cells by directly targeting TAGLN2. | ||
PLoS One Transgelin 2 participates in lovastatin-induced anti-angiogenic effects in endothelial cells through a phosphorylated myosin light chain-related mechanism.
| ||
Biochem Biophys Res Commun Transgelin-2 contributes to proliferation and progression of hepatocellular carcinoma via regulating Annexin A2. | ||
Molecules MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway |