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CoraLite®594-conjugated TNFR1 Monoclonal antibody

TNFR1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A6E3

Cat No : CL594-60192

Print datasheet

Synonyms

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

验证数据展示

  • IF Staining of human liver cancer using CL594-60192

    IF Staining of human liver cancer using CL594-60192

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 TNFR1 antibody (CL594-60192, Clone: 2A6E3 ) at dilution of 1:200.

  • IF Staining of human liver cancer using CL594-60192

    IF Staining of human liver cancer using CL594-60192

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 TNFR1 antibody (CL594-60192, Clone: 2A6E3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60192-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-60192 is TNFR1 Fusion Protein expressed in E. coli.

Tested Applications IF-P
Tested Reactivity human, mouse, rat, pig
Immunogen TNFR1 fusion protein Ag16112 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1A
Synonyms CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A
Calculated Molecular Weight 455 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC010140
Gene Symbol TNFRSF1A
Gene ID (NCBI) 7132
RRIDAB_2919901
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19438
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

实验方案

Product Specific Protocols
IF protocol for CL594 TNFR1 antibody CL594-60192Download protocol
Standard Protocols
Click here to view our Standard Protocols