- Featured Product
- KD/KO Validated
TNFR1 Monoclonal antibody
TNFR1 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A6E3
验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, HL-60 cells, Raji cells, Daudi cells, A549 cells, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human liver cancer tissue |
Positive FC detected in | Raji cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60192-1-Ig targets TNFR1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | FC, IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, FC, IHC |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | TNFR1 fusion protein Ag16112 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tumor necrosis factor receptor superfamily, member 1A |
Synonyms | CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A |
Calculated Molecular Weight | 455 aa, 50 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC010140 |
Gene Symbol | TNFRSF1A |
Gene ID (NCBI) | 7132 |
RRID | AB_10897160 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P19438 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for TNFR1 antibody 60192-1-Ig | Download protocol |
IHC protocol for TNFR1 antibody 60192-1-Ig | Download protocol |
IF protocol for TNFR1 antibody 60192-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Ther Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities. | ||
Front Immunol Single Cell Dissection of Epithelial-Immune Cellular Interplay in Acute Kidney Injury Microenvironment.
| ||
J Transl Med CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587. | ||
Life Sci Comparative transcriptome and Lipidome analyses suggest a lipid droplet-specific response to heat exposure of brown adipose tissue in normal and obese mice. | ||
Inflammation Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion. | ||
Oncotarget Inflammatory factor TNF-α promotes the growth of breast cancer via the positive feedback loop of TNFR1/NF-κB (and/or p38)/p-STAT3/HBXIP/TNFR1. |