• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated WWTR1 Monoclonal antibody

WWTR1 Monoclonal Antibody for IF-P, WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF-P, WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A12A10

Cat No : CL488-66500

Print datasheet

Synonyms

DKFZp586I1419, TAZ, WWTR1

验证数据展示

  • IF Staining of human liver cancer using CL488-66500

    IF Staining of human liver cancer using CL488-66500

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 TAZ antibody (CL488-66500, Clone: 2A12A10 ) at dilution of 1:200.

  • WB analysis using CL488-66500

    WB analysis using CL488-66500

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66500 (WWTR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MDA-MB-231 cells
Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66500-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66500 targets WWTR1 in WB, IF applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF-P, WB
Tested Reactivity Human, rat, mouse
Immunogen WWTR1 fusion protein Ag13330 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WW domain containing transcription regulator 1
Synonyms DKFZp586I1419, TAZ, WWTR1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZV5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 WWTR1 antibody CL488-66500Download protocol
IF protocol for CL Plus 488 WWTR1 antibody CL488-66500Download protocol
Standard Protocols
Click here to view our Standard Protocols