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  • Featured Product
  • KD/KO Validated

PRKACA Monoclonal antibody

PRKACA Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

4G10D7

Cat No : 67491-1-Ig

Print datasheet

Synonyms

PKA C alpha, PKACA, PRKACA

验证数据展示

  • WB analysis of HeLa using 67491-1-Ig

    WB analysis of HeLa using 67491-1-Ig

    WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

  • WB analysis using 67491-1-Ig

    WB analysis using 67491-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67491-1-Ig

    IHC staining of human breast cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included
  • IHC staining of human breast cancer using 67491-1-Ig

    IHC staining of human breast cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67491-1-Ig targets PRKACA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen PRKACA fusion protein Ag26400 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Synonyms PKA C alpha, PKACA, PRKACA
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC039846
Gene Symbol PRKACA
Gene ID (NCBI) 5566
RRIDAB_2882716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17612
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.

实验方案

Product Specific Protocols
WB protocol for PRKACA antibody 67491-1-IgDownload protocol
IHC protocol for PRKACA antibody 67491-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols