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PLA2G1B Monoclonal antibody

PLA2G1B Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C10

Cat No : 66397-1-Ig

Print datasheet

Synonyms

Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2

验证数据展示

  • WB analysis of pig pancreas using 66397-1-Ig

    WB analysis of pig pancreas using 66397-1-Ig

    pig pancreas tissue were subjected to SDS PAGE followed by western blot with 66397-1-Ig (PLA2G1B Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 66397-1-Ig

    IHC staining of human pancreas using 66397-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66397-1-Ig (PLA2G1B antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inpig pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66397-1-Ig targets PLA2G1B in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, pig
Immunogen PLA2G1B fusion protein Ag8812 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IB (pancreas)
Synonyms Group IB phospholipase A2, Phospholipase A2, PLA2, PLA2A, PLA2G1B, PPLA2
Calculated Molecular Weight70aa,8 kDa; 148aa,16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC005386
Gene Symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2881771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04054
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLA2G1B antibody 66397-1-IgDownload protocol
IHC protocol for PLA2G1B antibody 66397-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols