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CoraLite® Plus 488-conjugated NXF1 Monoclonal antibody

NXF1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C2H1

Cat No : CL488-67914

Print datasheet

Synonyms

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

验证数据展示

  • IF Staining of HEK-293 using CL488-67914

    IF Staining of HEK-293 using CL488-67914

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 NXF1 antibody (CL488-67914, Clone: 2C2H1 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-67914

    FC experiment of HEK-293 using CL488-67914

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NXF1 (CL488-67914, Clone:2C2H1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy NXF1 Ready-To-Use IHC Kit. NXF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67914-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67914 targets NXF1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen NXF1 fusion protein Ag27329 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear RNA export factor 1
Synonyms DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC004904
Gene Symbol NXF1
Gene ID (NCBI) 10482
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBU9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 NXF1 antibody CL488-67914Download protocol
FC protocol for CL Plus 488 NXF1 antibody CL488-67914Download protocol
Standard Protocols
Click here to view our Standard Protocols