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CoraLite® Plus 488-conjugated MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10302

Print datasheet

Synonyms

Atriopeptidase, CALLA, CD10, DKFZp686O16152, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

验证数据展示

  • FC experiment of Ramos using CL488-10302

    FC experiment of Ramos using CL488-10302

    1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MME,CD10 (CL488-10302)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10302-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10302 targets MME,CD10 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen MME,CD10 fusion protein Ag0427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms Atriopeptidase, CALLA, CD10, DKFZp686O16152, Enkephalinase, EPN, membrane metallo endopeptidase, MME, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC101658
Gene Symbol MME
Gene ID (NCBI) 4311
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08473
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 MME,CD10 antibody CL488-10302Download protocol
Standard Protocols
Click here to view our Standard Protocols