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MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10302-1-AP

Print datasheet

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

验证数据展示

  • WB analysis using 10302-1-AP

    WB analysis using 10302-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10302-1-AP (MME,CD10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 10302-1-AP

    IHC staining of human kidney using 10302-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • IHC staining of human kidney using 10302-1-AP

    IHC staining of human kidney using 10302-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10302-1-AP (MME,CD10 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CD10 antibody included
  • FC experiment of Jurkat using 10302-1-AP

    FC experiment of Jurkat using 10302-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (10302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue, human liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells, Ramos cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10302-1-AP targets MME,CD10 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, hamster
Immunogen MME,CD10 fusion protein Ag0427 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11
Calculated Molecular Weight 750 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC101658
Gene Symbol MME
Gene ID (NCBI) 4311
RRIDAB_10640893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
WB protocol for MME,CD10 antibody 10302-1-APDownload protocol
IHC protocol for MME,CD10 antibody 10302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Pathol

Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth - SYDE1 regulates placental cell migration and invasion.

Authors - Hsiao-Fan Lo
View Article
human,hamsterWB

Fitoterapia

A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ42 production via activating autophagy

Authors - Saijuan Li
View Article