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- KD/KO Validated
MIF Polyclonal antibody
MIF Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
FC, IF, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, U-937 cells, HL-60 cells, Y79 cells, rat spleen tissue, HEK-293T cells, HUVEC cells, Jurkat cells, U-87 MG cells |
| Positive IP detected in | mouse spleen tissue |
| Positive IF detected in | U-251 cells |
| Positive FC detected in | THP-1 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20415-1-AP targets MIF in WB, IP, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | FC, IF, IP, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MIF fusion protein Ag14058 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | macrophage migration inhibitory factor (glycosylation-inhibiting factor) |
| Synonyms | GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase |
| Calculated Molecular Weight | 115 aa, 12 kDa |
| Observed Molecular Weight | 12 kDa |
| GenBank Accession Number | BC000447 |
| Gene Symbol | MIF |
| Gene ID (NCBI) | 4282 |
| RRID | AB_10694820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14174 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIF antibody 20415-1-AP | Download protocol |
| IF protocol for MIF antibody 20415-1-AP | Download protocol |
| IP protocol for MIF antibody 20415-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.
| ||
Cell Prolif TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD). | ||
Front Endocrinol (Lausanne) Exercise prevents fatal stress-induced myocardial injury in obese mice | ||
Food Chem Toxicol The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation | ||
Mol Carcinog Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.
| ||
BMC Microbiol Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection. |
