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KD/KO Validated

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

283

Cat No : 15675-1-AP

Print datasheet

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

验证数据展示

WB analysis of HeLa cells using 15675-1-AP
WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.
WB analysis using 15675-1-AP
MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 15675-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human lung cancer using 15675-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD44 antibody included
IHC staining of human lung cancer using 15675-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD44 antibody included
IP experiment of HeLa using 15675-1-AP
IP result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.
FC experiment of HeLa using 15675-1-AP
1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human CD44 (15675-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

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Cat No. 15675-1-AP

Size

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经过测试的应用

Positive WB detected in	MDA-MB-231 cells, HeLa cells, HUVEC cells, A549 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells
Positive FC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 14 publications below
WB	See 189 publications below
IHC	See 76 publications below
IF	See 73 publications below
IP	See 1 publications below
FC	See 9 publications below
CoIP	See 2 publications below

产品信息

15675-1-AP targets CD44 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB,Neutralization,IP,IHC,IF,FC,CoIP
Tested Reactivity	human
Cited Reactivity	human, sika deer, Bovine
Immunogen	CD44 fusion protein Ag8143 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	CD44 molecule (Indian blood group)
Synonyms	CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I
Calculated Molecular Weight	742 aa, 82 kDa
Observed Molecular Weight	80-95 kDa
GenBank Accession Number	BC004372
Gene Symbol	CD44
Gene ID (NCBI)	960
ENSEMBL Gene ID	ENSG00000026508
RRID	AB_2076198
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P16070
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 15675-1-AP	Download protocol
IHC protocol for CD44 antibody 15675-1-AP	Download protocol
IF protocol for CD44 antibody 15675-1-AP	Download protocol
IP protocol for CD44 antibody 15675-1-AP	Download protocol
FC protocol for CD44 antibody 15675-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	IF	Nature ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis. Authors - Ting Zhang View Article
mouse	IF	Nat Commun Hyperthermia-triggered biomimetic bubble nanomachines Authors - Junbin Gao View Article
mouse	IF	Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss Authors - Yuxiang Hu View Article
human	WB,IHC,IF	Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma Authors - Yuantong Liu View Article
mouse	WB	Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy Authors - Chang Xiao View Article
human	WB	Biomaterials Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment Authors - Victor Passos Gibson View Article

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis of HeLa cells using 15675-1-AP

WB analysis using 15675-1-AP

IF Staining of HeLa using 15675-1-AP

IHC staining of human lung cancer using 15675-1-AP

IHC staining of human lung cancer using 15675-1-AP

IP experiment of HeLa using 15675-1-AP

FC experiment of HeLa using 15675-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis.

Hyperthermia-triggered biomimetic bubble nanomachines

The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy

Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment

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