- Featured Product
- KD/KO Validated
LGALS3BP Polyclonal antibody
LGALS3BP Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, COLO 320 cells, HEK-293 cells, fetal human brain tissue, HepG2 cells, A549 cells, human milk tissue, human plasma tissue |
Positive IP detected in | HepG2 cells, HEK-293 cells |
Positive IHC detected in | human oesophagus cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10281-1-AP targets LGALS3BP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | LGALS3BP fusion protein Ag0294 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lectin, galactoside-binding, soluble, 3 binding protein |
Synonyms | 90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K |
Calculated Molecular Weight | 585 aa, 65 kDa |
Observed Molecular Weight | 65-90 kDa |
GenBank Accession Number | BC002403 |
Gene Symbol | LGALS3BP |
Gene ID (NCBI) | 3959 |
RRID | AB_2137066 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q08380 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
IHC protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
IF protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
IP protocol for LGALS3BP antibody 10281-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. | ||
Cancer Res A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
| ||
Blood Adv Increased Mac-2 binding protein glycan isomer in patients at risk for late nonrelapse mortality after HSCT. | ||
PLoS Pathog Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex. | ||
Thromb Haemost Proteomic discovery of 21 proteins expressed in human plasma-derived but not platelet-derived microparticles. | ||
Cancer Sci MZT2A promotes NSCLC viability and invasion by increasing Akt phosphorylation via the MOZART2 domain. |