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- KD/KO Validated
Galectin-1 Polyclonal antibody
Galectin-1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, HCT 116 cells, NIH/3T3 cells, PC-12 cells, MCF-7 cells, U2OS cells |
| Positive IHC detected in | human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11858-1-AP targets Galectin-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Galectin-1 fusion protein Ag2432 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Synonyms | 14 kDa laminin binding protein, 14 kDa lectin, Gal 1, GAL1, Galaptin, Galectin 1, Galectin-1, HBL, HLBP14, HPL, Lactose binding lectin 1, LGALS1, S Lac lectin 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC020675 |
| Gene Symbol | LGALS1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_2234673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| IHC protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| IF protocol for Galectin-1 antibody 11858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells. | ||
Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. | ||
Biomed Pharmacother Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction. | ||
Int J Oncol Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer | ||
Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury. |
