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CoraLite® Plus 488-conjugated JAB1 Polyclonal antibody

JAB1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-27511

Print datasheet

Synonyms

COPS5, CSN5, JAB1, MOV 34, SGN5, Signalosome subunit 5

验证数据展示

  • FC experiment of HEK-293 using CL488-27511

    FC experiment of HEK-293 using CL488-27511

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JAB1 (CL488-27511) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COPS5/JAB1 Ready-To-Use IHC Kit. COPS5/JAB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27511-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27511 targets JAB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat
Immunogen JAB1 fusion protein Ag25950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
Synonyms COPS5, CSN5, JAB1, MOV 34, SGN5, Signalosome subunit 5
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC001187
Gene Symbol COPS5
Gene ID (NCBI) 10987
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92905
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 JAB1 antibody CL488-27511Download protocol
Standard Protocols
Click here to view our Standard Protocols