JAB1 Polyclonal antibody
JAB1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat and More (1)
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells, PC-12 cells |
Positive IHC detected in | mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27511-1-AP targets JAB1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | JAB1 fusion protein Ag25950 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis) |
Synonyms | COPS5, CSN5, JAB1, MOV 34, SGN5, Signalosome subunit 5 |
Calculated Molecular Weight | 37 kDa |
Observed Molecular Weight | 35-38 kDa |
GenBank Accession Number | BC001187 |
Gene Symbol | COPS5 |
Gene ID (NCBI) | 10987 |
RRID | AB_2880894 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92905 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for JAB1 antibody 27511-1-AP | Download protocol |
IHC protocol for JAB1 antibody 27511-1-AP | Download protocol |
IF protocol for JAB1 antibody 27511-1-AP | Download protocol |
FC protocol for JAB1 antibody 27511-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Oncol Identification of Key Transcription Factors and Immune Infiltration Patterns Associated With Breast Cancer Prognosis Using WGCNA and Cox Regression Analysis. | ||
Mol Carcinog PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma | ||
Cancer Cell Int The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells. | ||
Neuropsychiatr Dis Treat COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. | ||
Cell Commun Signal Alpha5 nicotinic acetylcholine receptor mediated immune escape of lung adenocarcinoma via STAT3/Jab1-PD-L1 signalling |