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IMMT Monoclonal antibody

IMMT Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D2E8

Cat No : 68226-1-Ig

Print datasheet

Synonyms

DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52

验证数据展示

  • WB analysis using 68226-1-Ig

    WB analysis using 68226-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68226-1-Ig (IMMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 68226-1-Ig

    IHC staining of mouse heart using 68226-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IMMT antibody included
  • IHC staining of mouse heart using 68226-1-Ig

    IHC staining of mouse heart using 68226-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 68226-1-Ig (IMMT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IMMT antibody included
  • IF Staining of HeLa using 68226-1-Ig

    IF Staining of HeLa using 68226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68226-1-Ig

    IF Staining of HeLa using 68226-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMMT antibody (68226-1-Ig, Clone: 2D2E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 68226-1-Ig

    FC experiment of HEK-293 using 68226-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IMMT (68226-1-Ig, Clone:2D2E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 68226-1-Ig

    WB analysis of HeLa using 68226-1-Ig

    WB result of IMMT antibody (68226-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMMT transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy IMMT Ready-To-Use IHC Kit. IMMT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68226-1-Ig targets IMMT in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Rat, Mouse
Immunogen IMMT fusion protein Ag0102 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name inner membrane protein, mitochondrial (mitofilin)
Synonyms DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52
Calculated Molecular Weight 90 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC002412
Gene Symbol IMMT
Gene ID (NCBI) 10989
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.

实验方案

Product Specific Protocols
WB protocol for IMMT antibody 68226-1-IgDownload protocol
IHC protocol for IMMT antibody 68226-1-IgDownload protocol
IF protocol for IMMT antibody 68226-1-IgDownload protocol
FC protocol for IMMT antibody 68226-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols