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ILK Polyclonal antibody

ILK Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24926-1-AP

Print datasheet

Synonyms

ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK

验证数据展示

  • IHC staining of human pancreas cancer using 24926-1-AP

    IHC staining of human pancreas cancer using 24926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 24926-1-AP

    IHC staining of human pancreas cancer using 24926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse heart using 24926-1-AP

    WB analysis of mouse heart using 24926-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 24926-1-AP

    IF Staining of MCF-7 using 24926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24926-1-AP (ILK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 24926-1-AP is ILK Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen ILK fusion protein Ag21494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin-linked kinase
Synonyms ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK
Calculated Molecular Weight 451 aa, 59 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC001554
Gene Symbol ILK
Gene ID (NCBI) 3611
RRIDAB_2879800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

实验方案

Product Specific Protocols
WB protocol for ILK antibody 24926-1-APDownload protocol
IHC protocol for ILK antibody 24926-1-APDownload protocol
IF protocol for ILK antibody 24926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

ACS Appl Mater Interfaces

Charge Reversal Polypyrrole Nanocomplex-Mediated Gene Delivery and Photothermal Therapy for Effectively Treating Papillary Thyroid Cancer and Inhibiting Lymphatic Metastasis.

Authors - Menghong Xu
View Article