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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FGFR3 Monoclonal antibody

FGFR3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F3G1

Cat No : CL594-66954

Print datasheet

Synonyms

ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4

验证数据展示

  • IF Staining of HepG2 using CL594-66954

    IF Staining of HepG2 using CL594-66954

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 FGFR3 antibody (CL594-66954, Clone: 1F3G1 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66954-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66954 targets FGFR3 in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen FGFR3 fusion protein Ag26290 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor receptor 3
Synonyms ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 125-135 kDa
GenBank Accession NumberNM_000142
Gene Symbol FGFR3
Gene ID (NCBI) 2261
RRIDAB_2920063
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3 and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, FGFR3 gene has also been associated with tumor progression.

实验方案

Product Specific Protocols
IF protocol for CL594 FGFR3 antibody CL594-66954Download protocol
Standard Protocols
Click here to view our Standard Protocols