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  • KD/KO Validated

FGFR3 Monoclonal antibody

FGFR3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G1

Cat No : 66954-1-Ig

Print datasheet

Synonyms

ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4



经过测试的应用

Positive WB detected inLNCaP cells, L02 cells, L-929 cells, HeLa cells, HEK-293 cells, HepG2 cells, A549 cells, NCI-H1299 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66954-1-Ig targets FGFR3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen FGFR3 fusion protein Ag26290 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibroblast growth factor receptor 3
Synonyms ACH, CD333, CEK2, FGFR 3, FGFR3, HSFGFR3EX, JTK4
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 125-135 kDa
GenBank Accession NumberNM_000142
Gene Symbol FGFR3
Gene ID (NCBI) 2261
RRIDAB_2882277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3 and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, FGFR3 gene has also been associated with tumor progression.

实验方案

Product Specific Protocols
WB protocol for FGFR3 antibody 66954-1-IgDownload protocol
IHC protocol for FGFR3 antibody 66954-1-IgDownload protocol
IF protocol for FGFR3 antibody 66954-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Ann Rheum Dis

FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.

Authors - Liang Kuang
  • KD Validated
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
mouseWB

Bioengineered

The effects of Omarigliptin on promoting osteoblastic differentiation.

Authors - Fake Liao
humanWB

J Exp Clin Cancer Res

HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.

Authors - Kai Guo
humanWB

J Exp Clin Cancer Res

Dysregulation and prometastatic function of glycosyltransferase C1GALT1 modulated by cHP1BP3/ miR-1-3p axis in bladder cancer.

Authors - Zengqi Tan
humanIF

Tissue Eng Part A

The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury

Authors - Limin Wang