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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated FBL Polyclonal antibody

FBL Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16021

Print datasheet

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1

验证数据展示

  • IF Staining of HeLa using CL488-16021

    IF Staining of HeLa using CL488-16021

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 FBL antibody (CL488-16021) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16021-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16021 targets FBL in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen FBL fusion protein Ag8841 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibrillarin
Synonyms FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1
Calculated Molecular Weight 321 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22087
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillarin (Fbl) is an essential nucleolar protein that plays an essential role in ribosome biogenesis and more particularly in the methylation of ribosomal RNAs and rDNA histones. FBL is highly expressed in various cancers and correlates with poor survival outcomes in cancer patients,such as hepatocellular carcinoma(PMID: 33376525). Knockdown of FBL sensitizes tumor cells and xenografts to DNA crosslinking agents, and leads to homologous recombination-mediated DNA repair defects(PMID: 37489617).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FBL antibody CL488-16021Download protocol
Standard Protocols
Click here to view our Standard Protocols