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  • KD/KO Validated

FBL Polyclonal antibody

FBL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16021-1-AP

Print datasheet

Synonyms

FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1



经过测试的应用

Positive WB detected inA375 cells, mouse liver tissue, mosue kidney tissue
Positive IP detected inA375 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FBL Ready-To-Use IHC Kit. FBL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16021-1-AP targets FBL in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FBL fusion protein Ag8841 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibrillarin
Synonyms FBL, FIB, FIB1, fibrillarin, FLRN, RNU3IP1
Calculated Molecular Weight 321 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC019260
Gene Symbol FBL
Gene ID (NCBI) 2091
RRIDAB_2105788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22087
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FBL antibody 16021-1-APDownload protocol
IHC protocol for FBL antibody 16021-1-APDownload protocol
IF protocol for FBL antibody 16021-1-APDownload protocol
IP protocol for FBL antibody 16021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice.

Authors - Xin Wang
  • KD Validated
humanWB,IF

Cell Biol Toxicol

SNORD17-mediated KAT6B mRNA 2'-O-methylation regulates vasculogenic mimicry in glioblastoma cells

Authors - Jingyi Cui
IF

Commun Biol

Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Authors - Tetsuya Yamamoto
humanWB

Oncotarget

Suppressor of fused (Sufu) represses Gli1 transcription and nuclear accumulation, inhibits glioma cell proliferation, invasion and vasculogenic mimicry, improving glioma chemo-sensitivity and prognosis.

Authors - Xing Liu
human,mouseWB

Front Immunol

RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.

Authors - Panpan Li
  • KD Validated
humanRIP

Mol Carcinog

SNORD60 promotes the tumorigenesis and progression of endometrial cancer through binding PIK3CA and regulating PI3K/AKT/mTOR signaling pathway

Authors - Wu Wu