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- KD/KO Validated
ETV5 Polyclonal antibody
ETV5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, Raji cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy ETV5 Ready-To-Use IHC Kit. ETV5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13011-1-AP targets ETV5 in WB, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ETV5 fusion protein Ag3708 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ets variant 5 |
| Synonyms | ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5 |
| Calculated Molecular Weight | 510 aa, 58 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC007333 |
| Gene Symbol | ETV5 |
| Gene ID (NCBI) | 2119 |
| RRID | AB_2278092 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41161 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETV5 antibody 13011-1-AP | Download protocol |
| IHC protocol for ETV5 antibody 13011-1-AP | Download protocol |
| IF protocol for ETV5 antibody 13011-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription. | ||
Nat Genet The dynamic, combinatorial cis-regulatory lexicon of epidermal differentiation. | ||
Nat Commun A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility | ||
Proc Natl Acad Sci U S A Transcription factor Etv5 is essential for the maintenance of alveolar type II cells. | ||
PLoS Biol Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis. |
