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CoraLite® Plus 488-conjugated EPHA7 Monoclonal antibody

EPHA7 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A7E5

Cat No : CL488-66667

Print datasheet

Synonyms

EHK 3, EHK3, EK11, EPH homology kinase 3, EPH like kinase 11, EPH receptor A7, EPHA7, Ephrin type A receptor 7, HEK11

验证数据展示

  • FC experiment of SH-SY5Y using CL488-66667

    FC experiment of SH-SY5Y using CL488-66667

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EPHA7 (CL488-66667, Clone:1A7E5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 66667-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66667 targets EPHA7 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen EPHA7 fusion protein Ag3813 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EPH receptor A7
Synonyms EHK 3, EHK3, EK11, EPH homology kinase 3, EPH like kinase 11, EPH receptor A7, EPHA7, Ephrin type A receptor 7, HEK11
Calculated Molecular Weight 998 aa, 112 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC027940
Gene Symbol EPHA7
Gene ID (NCBI) 2045
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15375
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. EPHA7 plays a role in corticogenesis processes, determines brain size and shape, and is involved in development of the central nervous system (PMID:34176129). In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor. It can participate in cell proliferation, migration and apoptosis through various mechanisms, and afect tumor diferentiation, staging and prognosis. EphA7 may be a potential diagnostic marker and tumor treatment target (PMID:35112312).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 EPHA7 antibody CL488-66667Download protocol
Standard Protocols
Click here to view our Standard Protocols