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  • Featured Product
  • KD/KO Validated

EIF2A/CDA02 Monoclonal antibody, PBS Only

EIF2A/CDA02 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G7H4

Cat No : 66482-1-PBS

Print datasheet

Synonyms

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089

验证数据展示

  • WB analysis of HEK-293 using 66482-1-Ig (same clone as 66482-1-PBS)

    WB analysis of HEK-293 using 66482-1-Ig (same clone as 66482-1-PBS)

    WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

    IHC staining of human prostate cancer using 66482-1-Ig (same clone as 66482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66482-1-Ig (same clone as 66482-1-PBS)

    IF Staining of HepG2 using 66482-1-Ig (same clone as 66482-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66482-1-Ig (EIF2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

  • WB analysis using 66482-1-Ig (same clone as 66482-1-PBS)

    WB analysis using 66482-1-Ig (same clone as 66482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66482-1-PBS in a different storage buffer formulation.

产品信息

66482-1-PBS targets EIF2A/CDA02 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity Human, mouse, rat
Immunogen EIF2A/CDA02 fusion protein Ag22041 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2A, 65kDa
Synonyms CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BY44
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.