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  • Featured Product
  • KD/KO Validated

EIF2A/CDA02 Monoclonal antibody

EIF2A/CDA02 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7H4

Cat No : 66482-1-Ig

Print datasheet

Synonyms

CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089

验证数据展示

  • WB analysis of HEK-293 using 66482-1-Ig

    WB analysis of HEK-293 using 66482-1-Ig

    WB result of EIF2A/CDA02 antibody (66482-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2A/CDA02 transfected HEK-293 cells.

  • IHC staining of human prostate cancer using 66482-1-Ig

    IHC staining of human prostate cancer using 66482-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66482-1-Ig (EIF2A antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66482-1-Ig

    IF Staining of HepG2 using 66482-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66482-1-Ig (EIF2A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66482-1-Ig

    WB analysis using 66482-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66482-1-Ig (EIF2A/CDA02 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inA431 cells, ROS1728 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

66482-1-Ig targets EIF2A/CDA02 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen EIF2A/CDA02 fusion protein Ag22041 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2A, 65kDa
Synonyms CDA02, EIF 2A, EIF2A, EIF2A/CDA02, MST089, MSTP004, MSTP089
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011885
Gene Symbol EIF2A
Gene ID (NCBI) 83939
RRIDAB_2881848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BY44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2A, also named as CDA02, MSTP004 and MSTP089, belongs to the WD repeat EIF2A family. It functions in the early steps of protein synthesis of a small number of specific mRNAs. EIF2A, an alternative initiator tRNA-binding protein, has a key role in the translation of HCV mRNA during HCV infection, in turn promoting eIF2a phosphorylation by activating the eIF2a kinase PKR.

实验方案

Product Specific Protocols
WB protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IHC protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
IF protocol for EIF2A/CDA02 antibody 66482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharm Biol

Cinobufagin induces acute promyelocytic leukaemia cell apoptosis and PML-RARA degradation in a caspase-dependent manner by inhibiting the β-catenin signalling pathway

Authors - Yaoyao Bian
View Article
humanWB

Front Neurosci

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease

Authors - Fahad Mubariz
View Article