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CoraLite®594-conjugated DDX54 Monoclonal antibody

DDX54 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9F4

Cat No : CL594-66664

Print datasheet

Synonyms

DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97

验证数据展示

  • IF Staining of PC-3 using CL594-66664

    IF Staining of PC-3 using CL594-66664

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite®594 DDX54 antibody (CL594-66664, Clone: 1H9F4 ) at dilution of 1:200.

  • FC experiment of PC-3 using CL594-66664

    FC experiment of PC-3 using CL594-66664

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human DDX54 (CL594-66664, Clone:1H9F4) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inPC-3 cells
Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 66664-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66664 targets DDX54 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse
Immunogen DDX54 fusion protein Ag25289 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Synonyms DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC156669
Gene Symbol DDX54
Gene ID (NCBI) 79039
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDD1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

实验方案

Product Specific Protocols
IF protocol for CL594 DDX54 antibody CL594-66664Download protocol
FC protocol for CL594 DDX54 antibody CL594-66664Download protocol
Standard Protocols
Click here to view our Standard Protocols