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DDX54 Monoclonal antibody

DDX54 Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

FC, IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1H9F4

Cat No : 66664-1-Ig

Print datasheet

Synonyms

DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97

验证数据展示

  • WB analysis using 66664-1-Ig

    WB analysis using 66664-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IF Staining of PC-3 using 66664-1-Ig

    IF Staining of PC-3 using 66664-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 66664-1-Ig

    WB analysis using 66664-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66664-1-Ig (DDX54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 66664-1-Ig

    IF Staining of PC-3 using 66664-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DDX54 antibody (66664-1-Ig, Clone: 1H9F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of PC-3 using 66664-1-Ig

    FC experiment of PC-3 using 66664-1-Ig

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug Anti-Human DDX54 (66664-1-Ig, Clone:1H9F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, PC-3 cells, U-251 cells, U-87 MG cells
Positive IF detected inPC-3 cells
Positive FC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66664-1-Ig targets DDX54 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF/ICC, WB, ELISA
Cited ApplicationsIHC, WB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen DDX54 fusion protein Ag25289 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Synonyms DDX54, DEAD box protein 54, DEAD box RNA helicase 97 kDa, DP97
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC156669
Gene Symbol DDX54
Gene ID (NCBI) 79039
RRIDAB_2882019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX54, also named as ATP-dependent RNA helicase DDX54, is a 881 amino acid protein, which contains 1 helicase ATP-binding domain and belongs to the DEAD box helicase family. DDX54/DBP10 subfamily. DDX54 localizes in nucleus and Interacts in a hormone-dependent manner with nuclear receptors. DDX54 has RNA-dependent ATPase activity and represses the transcriptional activity of nuclear receptors.

实验方案

Product Specific Protocols
WB protocol for DDX54 antibody 66664-1-IgDownload protocol
IF protocol for DDX54 antibody 66664-1-IgDownload protocol
FC protocol for DDX54 antibody 66664-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

DDX54 Plays a Cancerous Role Through Activating P65 and AKT Signaling Pathway in Colorectal Cancer.

Authors - Yi Yu
View Article