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CoraLite® Plus 488-conjugated Connexin 43 Recombinant antibody

Connexin 43 Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3O6

Cat No : CL488-80543

Print datasheet

Synonyms

Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

验证数据展示

  • IF Staining of mouse heart using CL488-80543

    IF Staining of mouse heart using CL488-80543

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Connexin 43 antibody (CL488-80543, Clone: 3O6 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 80543-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80543 targets Connexin 43 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF-P
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Connexin 43 fusion protein Ag25533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gap junction protein, alpha 1, 43kDa
Synonyms Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC026329
Gene Symbol GJA1
Gene ID (NCBI) 2697
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17302
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Connexin 43 antibody CL488-80543Download protocol
Standard Protocols
Click here to view our Standard Protocols