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Connexin 43 Recombinant antibody, PBS Only

Connexin 43 Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O6

Cat No : 80543-1-PBS

Print datasheet

Synonyms

Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

验证数据展示

  • WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

    WB analysis using 80543-1-RR (same clone as 80543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80543-1-RR (Connexin 43 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IHC staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 80543-1-RR (Connexin 43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    IF Staining of mouse heart using 80543-1-RR (same clone as 80543-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Connexin 43 antibody (80543-1-RR, Clone: 3O6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80543-1-PBS in a different storage buffer formulation.

产品信息

80543-1-PBS targets Connexin 43 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Connexin 43 fusion protein Ag25533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gap junction protein, alpha 1, 43kDa
Synonyms Connexin 43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC026329
Gene Symbol GJA1
Gene ID (NCBI) 2697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17302
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Connexin 43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Connexin 43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).