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CoraLite® Plus 488-conjugated Cd68 Polyclonal antibody

Cd68 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-28058

Print datasheet

Synonyms

Cd68, CD68 antigen, gp110, Scard1

验证数据展示

  • IF Staining of mouse spleen using CL488-28058

    IF Staining of mouse spleen using CL488-28058

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

  • IF Staining of mouse spleen using CL488-28058

    IF Staining of mouse spleen using CL488-28058

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 Cd68 antibody (CL488-28058) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28058-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28058 targets Cd68 in IF applications and shows reactivity with mouse samples.

Tested Applications IF-P
Tested Reactivity mouse
Immunogen Cd68 fusion protein Ag27827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD68 antigen
Synonyms Cd68, CD68 antigen, gp110, Scard1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberNM_001291058
Gene Symbol Cd68
Gene ID (NCBI) 12514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31996
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cd68 antibody CL488-28058Download protocol
Standard Protocols
Click here to view our Standard Protocols