Cd68 Polyclonal antibody
Cd68 Polyclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (3)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
184
验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse spleen tissue |
| Positive FC detected in | RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28058-1-AP targets Cd68 in WB, IF, FC, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat, pig, canine |
| Immunogen | Cd68 fusion protein Ag27827 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD68 antigen |
| Synonyms | Cd68, CD68 antigen, gp110, Scard1 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | NM_001291058 |
| Gene Symbol | Cd68 |
| Gene ID (NCBI) | 12514 |
| RRID | AB_2881049 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31996 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cd68 antibody 28058-1-AP | Download protocol |
| IHC protocol for Cd68 antibody 28058-1-AP | Download protocol |
| IF protocol for Cd68 antibody 28058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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