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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CXCR3B-specific Monoclonal antibody

CXCR3B-specific Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2D6

Cat No : CL488-60065

Print datasheet

Synonyms

CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR

验证数据展示

  • FC experiment of U-937 using CL488-60065

    FC experiment of U-937 using CL488-60065

    1X10^6 U-937 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CXCR3B-specific (CL488-60065, Clone:1B2D6) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inU-937 cells
For other applications, we recommend the unconjugated version of this antibody, 60065-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60065 targets CXCR3B-specific in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 3
Synonyms CD183, CKR L2, CMKAR3, CXC R3, CXCR 3, CXCR3, CXCR3A, CXCR3B, CXCR3B-specific, G protein coupled receptor 9, GPR9, IP 10 receptor, IP10 R, Mig R, MigR
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40 kDa+43 kDa
GenBank Accession NumberAF469635
Gene Symbol CXCR3
Gene ID (NCBI) 2833
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49682
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CXCR3B-specific antibody CL488-60065Download protocol
Standard Protocols
Click here to view our Standard Protocols