• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 647 Anti-Mouse CD103 (2E7)

CD103 Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E7

Cat No : CL647-65047

Print datasheet

Synonyms

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

验证数据展示

  • FC experiment of BALB/c mouse splenocytes using CL647-65047

    FC experiment of BALB/c mouse splenocytes using CL647-65047

    1X10^6 BALB/c mouse splenocytes were surface stained with 5 ul CoraLite®488 Anti-Mouse CD3 (CL488-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Armenian Hamster IgG isotype control (left) or 5 ul CoraLite® Plus 647 Anti-Mouse CD103 (CL647-65047, Clone:2E7) (right). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65047-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65047 targets CD103 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL) 种属同源性预测
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Full Name integrin alpha E, epithelial-associated
Synonyms A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae
GenBank Accession NumberBC150690
Gene Symbol Itgae
Gene ID (NCBI) 16407
RRIDAB_2883652
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ60677
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD103 antibody CL647-65047Download protocol
Standard Protocols
Click here to view our Standard Protocols