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CoraLite® Plus 488-conjugated CCT5 Monoclonal antibody

CCT5 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12E10

Cat No : CL488-67400

Print datasheet

Synonyms

CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon

验证数据展示

  • IF Staining of HepG2 using CL488-67400

    IF Staining of HepG2 using CL488-67400

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CCT5 antibody (CL488-67400, Clone: 1B12E10 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67400

    FC experiment of HepG2 using CL488-67400

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CCT5 (CL488-67400, Clone:1B12E10) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67400-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67400 targets CCT5 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen CCT5 fusion protein Ag21421 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Synonyms CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon
Calculated Molecular Weight 541 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC035499
Gene Symbol CCT5
Gene ID (NCBI) 22948
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CCT5 antibody CL488-67400Download protocol
FC protocol for CL Plus 488 CCT5 antibody CL488-67400Download protocol
Standard Protocols
Click here to view our Standard Protocols