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CoraLite®594-conjugated Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, canine

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4C12A6

Cat No : CL594-66020

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

验证数据展示

  • IF Staining of HeLa using CL594-66020

    IF Staining of HeLa using CL594-66020

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66020 (Bcl-XL antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL594-66020

    FC experiment of HeLa using CL594-66020

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (CL594-66020, Clone:4C12A6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells, MDCK cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66020 targets Bcl-XL in IF, FC (Intra) applications and shows reactivity with human, canine samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, canine
Immunogen Bcl-XL fusion protein Ag18037 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC019307
Gene Symbol BCL2L1
Gene ID (NCBI) 598
RRIDAB_2883480
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

实验方案

Product Specific Protocols
IF protocol for CL594 Bcl-XL antibody CL594-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols