Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat No : 66020-1-Ig

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL



经过测试的应用

Positive WB detected inJurkat cells, A431 cells, HeLa cells, Ramos cells, K-562 cells, Raw 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue, human testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66020-1-Ig is Bcl-XL Fusion Protein expressed in E. coli.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Bcl-XL fusion protein Ag18037 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, Bcl-XL
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC019307
Gene Symbol BCL2L1
Gene ID (NCBI) 598
RRIDAB_11042315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

实验方案

Product Specific Protocols
WB protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IHC protocol for Bcl-XL antibody 66020-1-IgDownload protocol
IF protocol for Bcl-XL antibody 66020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
humanWB

J Immunother Cancer

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells

Authors - Songbo Zhao
humanWB

Acta Pharmacol Sin

Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells

Authors - Wa Ding
humanWB

Blood Cancer J

The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.

Authors - Xinan Qiao
humanWB

Antioxid Redox Signal

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Authors - Tingting Ren
humanWB

J Med Chem

Design, Synthesis, and Activity Evaluation of Novel Acyclic Nucleosides as Potential Anticancer Agents In Vitro and In Vivo.

Authors - Er-Jun Hao